168 J. Wyman on living organisms in heated water. 
would be the same in both series, as it was in the aoe 
experiments, the only difference being that the quantity woul 
be less, and there would be none which had not been boiled. 
. XVITI.—Nineteen flasks of about 75 c. c. capacity, each 
containing about 15.¢.c. of boiled and filtered beef juice were 
divided into two series, one of nine and the other of ten flasks. 
All of series I were sealed at the temperature of the room, 
without any addition to their contents. After.a drop of water 
containing active infusoria had been added to each of series I, 
these were also sealed at the temperature of the room, and 
all of both series boiled 15 minutes. 
They were allowed to stand twelve days in a cold room in the 
winter, when no change having taken place they were removed 
to a room in which the temperature ranged from 50° to 70° F, 
day and night. Six days later, on the 18th from the beginning 
of the experiment, five flasks of each series had become turbid, 
and on the 20th all had become so. There was no obvious dif 
ference between the two series in the time required for the de- 
velopment of the infusoria. A longer time passed before the 
infusoria appeared than in the experiments with the open bot 
tles, but this is to be attributed in part to the coldness of the 
room in which they were at first placed. But even in the warm 
room it was six days before they became turbid. 
zp. XIX.—Seven flasks containing boiled and filtered beef 
juice were divided into two series—I, consisting of three flasks, 
had added to the above fluid in each one drop of infusorial 
fluid, and were sealed and allowed to remain at the temperature 
of the room. Series II consisted of four flasks prepared as 12 
series I, but after being sealed were boiled 30 minutes. , 
At the end of 18 hours two of series I had become turbid 
but none of II. At the 25th hour all of I were turbid and tw? 
) On the 8d day three of II were turbid and on the 4th 
were. 
Exp. XX.—A fluid was prepared containing half a gram of 
extract of beef to 100c.c. of water. Twenty-one flasks co? 
taining this fluid were divided into four series. ‘ai 
Sertss IJ, consisting of three flasks which were sealed at the 
of dust from. the si of shelves, to about 200 c. c. of boiled wa 
V sled; the mixture was shaken, the heaviest 
particles of dust allowed to settle, and the rest poured off, At 
the end of a few days this fluid contained active Vibrios. 
RIES consisted of si 
six flasks containing the same as those 
of series I, sealed at the te ture of th and 
a ee mperature e room, 
Sertzs IV, consi ing of six flasks, k: : contained the same fluid 
