J. Wyman on living organisms in heated water. 169 
with the addition of one drop of dust water to each flask; all 
were boiled 30 minutes. 
_On the second day one of series I, and all those of II were tur- 
bid and had a film—III and IV were unchanged, and perfect] 
transparent. On the third day infusoria appeared in III and IV, 
but were confined to the surface, forming a granular looking 
film, the fluid remaining transparent. 
‘The contents of the flasks were examined with the microscope. 
III and IV also contained Vibrios, but of a very minute size and 
moved with great rapidity. 
The boiling in the above experiment was followed by a later 
appearance of infusoria in IJ and IV than inI and I. The 
addition of dust water to series II, hastened the development of 
infusoria, In series IV to which the same dust had been added, 
but the flask and contents subsequently boiled after being sealed, 
Infusoria appeared one day later. 
Conclusions.—The following conclusions appear to the writer 
ea justified by the observations and experiments recorded in 
Ig paper. 
Ist. In thermal waters plants belonging to the lower kinds of 
Alge live in water the temperature of which in some instances 
Mises as high as 208° F. 
2d. Solutions of organic matter boiled for twenty-five minutes, 
and exposed only to air which had passed through iron tubes 
heated to redness, became the seat of infusorial life. HExps.I-V. 
8d. Similar solutions contained in flasks hermetically sealed, 
and then immersed in boiling water for periods varying from a 
few minutes to four hours, also became the seat of infusorial life. 
_ — were chiefly Vibrios, Bacteriums and Monads, 
Ps. VI-XYV, 
. 4th. No ciliated infusoria, unless Monads are such, appeared 
in the experiments referred to in the above conclusions. 
5th. No infusoria of any kind appeared if the boiling was pro- 
longed beyond a period of five hours. : 
6. Tnfusoria having the faculty of locomotion lost this when 
in water to a temperature of from 120° to 134° F. Exp. 
__1. If Vibrios, Bacteriums and Monads are added to a clear 
and limpid organic solution, this becomes turbid from their mul- 
“plication fei Sat one to two days. If however they have been 
Fviousl y boiled, the solution does not become turbid, ntil 
™ one to two days later, and in some of the experiments not 
Oner than does the same solution to which no infusoria have 
& Au. Jour. Sci.—Srconp Seria, Vou. XLIV, No. 131, ee ie 
