Geology and Natural History. 385 
Prof. Huizinga begins his paper with the words Mudta renas- 
centur que jam cecidere, using them as an expression of the recur- 
ing nature of this question. He then proceeds to say that he was 
induced to undertake his inquiry by the publication of the well- 
known work of Dr. Bastian (whom he compliments as having 
awakened the exhausted interest of physiologists in the subject), 
has thought it necessary to repeat the experiment under what he 
Tegards as conditions of greater exactitude. : 
Huizinga’s objections to Bastian’s experiment are two. First, 
that when a flask is boiled and closed ermetically in ebullition, 
its contents are almost entirely deprived of air, and (2) that — 
j iti viate 
e 
hot porcelain plate is made to adhere to the edge or lip of the flask 
bya layer of asphalt, with which the edge has been previously 
the flask, at the same time that all germinal matter is excluded. 
t necessary to discuss whether this is so or not. — 
: To obviate the second objection he alters the composition of the 
liquid used; he substitutes for cheese, peptone, and for turni 
infusion, a solution containing, in a liter of distilled water— 
Ae i . - 25 grams. 
2 “ 
n ‘ 
Magnesium sulphate, - - ‘ - 2 ag 
alcium Pp osp ate, seg es & Oo ‘“ 
The phosphate is prepared by precipitating a solution of a 
dina i i 
oiling, i , 
Phosphate, and insoluble basic salt, of which the latter is removed 
i infiltration, Consequently the proportion of phosphate in solu- 
‘7, s te8s than that above indicated 
