28 R. H. Chittenden— Glycogen and Glycocoll in the 
The substance is insoluble in alcohol and ether, has no reduc- 
ing action with cupric sulphate and sodium hydroxide, but when 
boiled with a few drops of dilute hydrochloric acid gives a clear 
fluid which has strong reducing action. This same reaction takes 
place also with nitric and sulphuric acids, but not so readily as 
with the former. A portion of the substance was treated with - 
a small quantity of saliva at the ordinary temperature, and at 
40° C., and in both cases the ptyalin acted immediately upon it 
and sugar was formed. Treated with a solution of iodine in 
potassium iodide, a brownish red or maroon color was obtained. 
These and other reactions pointed to glycogen. It was yet to 
be ascertained whether the sugar oot by the action of acids 
and ferments was aad also to examine the action of boiling 
dilute nitric acid upon it, and to determine whether the different 
formule of glycogen could be obtained by drying it at different 
mperatures. A portion of the substance was then boiled 
with hydrochloric acid not alcohol produced no precipitate in 
a sample tested, the excess of acid removed by oxide of silver 
and the sugar obtained oe evaporation. The product had all 
the properties of glucose, was intensely sweet, reduced alkaline 
solutions of copper rand silver and yielded Pettenkofer’s reaction. 
Analyzed, it gave the following result: 
Calculated. 
2 C;H,.0;+H,0 
C 3615 36°17 36°36 
H 6°82 6°88 7:07 
O 57°03 56°59 56°57 
By the action of boiling dilute nitric acid, oxalic acid was 
ott and separated. A different sample of the original sub- 
stance, dried over sulphuric acid until a constant weight was 
eamel, yielded : 
1. 2. 
C 43°81 43°90 
H 6°43 6°46 
O 49°76 49°64 
A sample dried at 100° C. gave by analysis: 
1. _ Analysis of starch dried at 100° C. by Mulder’ 
C 43°86 43°89 43°86 
H 6°41 6°38 6°28 
O 49°73 49°73 
A sample dried at 140° C.: 
2, Analysis of starch dried at 140° by Mulder 
44°47 
Cc aaa 44°40 
H 6°38 6°41 6°28 
O 49°30 49-19 
On treating an aqueous solution of the substance at the ordi- 
temperature with an excess of a saturated solution of 
