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64 G. F. Barker—Physiological Chemistry. 
to be tested is added, and the ebullition continued for three 
minutes, If at the end of this time, no reaction takes place, the 
absence of sugar may be assumed. With six healthy rabbits, ex- 
periments conducted in this way gave a negative result through- 
out; hence not a trace of sugar exists in the livers of these ani- 
the other by Ritter’s mode. In some instances the latter 
process was at once applied to the piece first taken. In every 
case, the piece of liver treated with boiling water gave the 
reaction for sugar, while the piece prepared by trituration with 
alcohol, never afforded the slightest trace of sugar. Hulen- 
burg differs also from Ritter on the effect of narcosis on the pro- 
duction of sugar. He shows that the ether-narcosis, at least, 
when not carried too far, does not cause the appearance of sugar 
in the liver, by the following experiments : two powerful albino 
rabbits inhaled pure sulphuric ether for 1 or 14 minutes to com- 
plete insensibility to mechanical irritation, when the abdomen 
was opened, a piece of the liver removed and examined as usual. 
In both animals, the liver was entirely free from sugar, as also 
was the urine. After the death of the animals, however, the 
liver reduced readily the copper-test. A third rabbit was nar- 
cotized with carbonic tetrachlorid, until serious disturbances of 
the respiration, facial breathing and dispnoetic convulsions ap- 
peared, At this stage, the animal-seemed dead ; the abdomen 
was opened—the heart pulsating regularly—and a piece of the 
liver removed, and found to contain sugar e urine was also 
saccharine. “It appears therefore, that only a narcotization by 
inhalation rising to a fatal, or at any rate to an extraordinarily 
intense intoxication, can cause the ante-mortem production of 
sugar in the liver.” In conclusion, Eulenburg maintains with 
Ritter, that “even instantly after death, a fragment of the liver, 
pets ied triturated with alcohol, shows itself weed 
sacc 
tion contains 150 grams commercial Reg obras should not contain more than 
10 per cent of hygroscopic cae we e. Porta ach experiment, the 
three fluids mixed i 
appears, the solution may be used for testing; if it is troubled, the tartaric acid 
solution must be prepared anew.—Jahresbericht, 1854, p. 747. 
