82 Dr. J. Wyman— Experiments on Infusoria. 



pepper was boiled in a flask of 250 c. c. capacity. It was opened 

 on the 20th day, but no living organisms were found. 



Exp. V. (1.) Feb. 5th. A solution of sugar, gelatine, and 

 cheese was boiled and filtered, and again boiled in the flask, 

 which was opened on the 29th day, and no organisms detected. 



Exp. VI. (2.) Feb. 10th. A solution of gelatine and sugar to 

 which was added a few drops of urine and milk, were put into a 

 bolt-bead, the tube of which had been drawn to a neck, and after 

 boiling, was hermetically sealed. The flask was opened on the 

 13th day and found to contain yeast plants and some very slen- 

 der filaments which appeared to be of a vegetable nature. 



Exp. VII. (1.) Feb. 10th. Twenty cubic centimetres of a 

 solution like the preceding was boiled in a flask of 875 c.c. 

 capacity. A film formed on the 11th day, and on the 30th the 

 flask was opened and found to contain Vibrios and Bacteriums. 



Exp. vin. (1.) Feb. 25th. A solution of sugar and gelatine 

 to which fragments of green leaves and flesh were added, was 

 boiled Ih and 40™. The flask was opened on the 15th day ; no 

 organisms were found. 



Exp. IX. (1.) Feb. 25th. The same as the preceding, without 

 the addition of the flesh ; this solution was boiled 40' and opened 



I the third day ; no organisms were found. 



Exp. X (1.) March. 6th. Three flasks, a b c, were prepared 

 in the same way, each containing a solution of sugar and gelatine 

 to which was added a few drops of urine and some fragments of 

 muscle ; a and c were boiled 30' and h 1^. Air was supplied to 

 a and h through a heated tube and to c at the temperature of the 

 room. A film formed in a on the 11th and in c on the twelfth 

 day, and at a later period in h. They were all opened a few days 

 afterwards and found to contain Bacteriums, Vibrios and ferment 

 cells. 



Exp. XL (1.) March 12th. An ounce of meat was suspend 



- . ■; " !.c. of wat 



This was boiled 20', duringVhich tin 



flask of 850 cub. cent, capacity with about 40 c. c. of water 

 in it. This was boiled 20', during which time the meat was ex- 

 posed to the steam in the flask. The juice which dropped from 

 the meat was coagulated in the water beneath, and the meat itself 

 was thoroughly cooked ; on the second day the meat was covered 

 with a gelatinous exudation, and on the third a film was formed 

 on the surface of the water. The flask was opened on the fifth 

 day and found to contain Vibrios, Bacteriums, and a few fer- 

 ment cells. The gelatinous exudation on the surface of the meat 

 also contained the same organisms, and appeared to be wholly 

 made up of them. 



Exp. xii. (2.) March 13th. The juice of an ounce of beef, to 

 which was added 10 cub. cent of urine and 40 c. c. of water w^ 

 boiled 20' in a bolt-head and hermetically sealed. A film formed 

 on the 4th, and the flask was opened on the llth day, when there 

 was a distinct rush of air outwards. Large numbers of Bacte- 



