Exp 



Dr. J. Wyman— Experiments on Infusoria. 83 



riums were found, also small spherical bodies with ciliary motions 

 and oval bodies like Kolpoda, containing what appeared to be 

 Bacteriums ; one of these Kolpoda-like bodies moved with cilia. 



Exp. XIII. (1.) March loth. An ounce of beef was suspended 

 over water in a flask of 500 c. c. capacity and boiled 25'. The 

 same changes took place as in the preceding experiment. The 

 exudation appeared on the surface of the meat, and the film on 

 the water on the 2d day ; on the 4th day the meat fell to pieces ; 

 the flask was opened on the 9th day; Bacteriums were found in 



?e numbers and there was a slight odor of putrefaction. 



5xp. XIV. (1.) March 17th. Juice of beef and a few shreds of 

 oeef were boiled in a flask of 300 cub. cent, capacity for 20'. A 

 film was formed on the 2d day and the flask was opened on the 

 11th day. Bacteriums existed in abundance. 



Exp. XV. (1.) March 19th. Fifty cubic centimetres of beef 

 were boiled 40' in a flask of 800 c. c. capacity. The film began 

 to form on the 2d day, and the flask was opened on the 24th. 

 The film had disappeared, the fluid had a nauseous odor and the 

 Bacteriums were diffused through the whole mass instead of 

 being mostly confined to the surface as in the preceding experi- 

 ments. . 



Expts. XVI, XVII XVIII, XIX, (2.) March 20th, were made with 

 jnice of beef and water in flasks of 550 cub. cent, capacity ;xvi 

 was boiled 15', the film formed on the second day and the flask 

 was opened on the 9th. Vibrios were found in abundance, of 

 different lengths, some of them moving with great rapidity, 

 xvii was boiled 30', the film was formed on the 3d and the flask 

 was opened on the 9th day. Vibrios were found in great num- 

 bers, some of them bending and extending themselves rapidly 

 Some minute spherical bodies were also seen, having the kind of 

 inotion which results from vibrating cilia, though none of these 

 were detected, xviii was boiled 15', the fluid having been pre- 

 viously filtered: the film formed on the 8d, and the flask was 

 opened on the 8th day ; the organisms found were the same as 

 ^[^ -^vii. xix was boiled 1". The film formed on the 2d and 

 tile flask was opened on the 24th day. The infusion had a 

 sl'v^iuly putrid odor and contained Vibrios and Bacteriums. 



^xp. XX. (2.) March 22. The flask and the contents of it 

 ^ere the same as in the experiments just described. The solu- 

 ' 3 boiled 15'. The film formed on the 5th, and the flask 



^as opened on the 31st day. The fluid had become of a dark 

 reddish brown color, the film had disappeared, and some ot the 

 s^hreds of the coacrulated albumen had become nearly black, 

 bacteriums were found in large numbers, and the darker shreds 

 seemed to be made up of them. . ^ •, ^ .a, .-u 



Exp. XXI. (2.) March 27th. Beef juice was boiled 40' ; the 

 flask was opened on the 25th day, and found to contain Bac- 



