84 Dr. J. Wyman — Experiments on Infusoria. 



Exp. XXII. (2.) April 2d. Beef juice and fragments of beef 

 were boiled 15', and tbe air was introduced through a much 

 smaller tube. Bacteriums were found on the 20th day. 



Exp. XXIII, (2,) April 5th, was prepared in the same way a3 

 the preceding experiment. The lilm formed on the 6th day, 

 and the flask was opened on the 17th. The sealed end waa 

 melted in the flame of a spirit lamp, when the gas escaped with 

 force. Bacteriums were found. 



Expts. XXIV, XXV, XXVI, (3,) were all prepared in the same 

 way, April 16th. The capacity of the flasks was 550 c. c. ; the 

 contents were beef juice and water 17 c. c, urine 7 c. c. The 

 flasks were folded in a napkin, immersed in water, which was 

 gradually heated to the boiling point, and each then exposed to 

 it for 30'. The film formed in xxvi on the 4th day, and in 

 sxiv and xxv on the 5th, and were all subsequently found to 

 contain Bacteriums. 



Expts. XXVII, XXVIII. (3). April 24th. Two flasks, each of 

 550 c. c. capacity, and each containing about 20 c. c. of beef 

 juice and urine, were hermetically sealed at the temperature of 

 the room, wrapped in cloth, and exposed for two hours to boil- 

 ing water. The film formed on the 4th day ; one of them was 

 opened on the 5th and the other on the llth, and both found 

 to contain Bacteriums. 



Expts. XXIX, XXX. (1.) February 17th. In both of these the 

 contents of the flasks were solutions of sugar and gelatine ui 

 water, to which fragments of cabbage leaves were added. Tlie 

 air was introduced through a Bohemian glass tube, filled with 

 asbestos and platinum sponge, and heated to redness. The 

 materials were boiled 30'. In xxix the film was formed on the 

 29th, and the flask was opened on the 39th day. The solution 

 was found to contain Bacteriums and cells filled with them. la 

 XXX the film was formed on the 7th day, and Bacteriums were 

 found on the 23d, when there was a slight odor of putreflictio;i. 



Expts. XXXI, XXXII, XXXIII. (2.) March 24th. 30 grains ut 

 sugar, 20 c. c. of beef juice, 158 c. c. of water, were divided ir/- 

 three parts, and each part put into a flask of 550 c. c. cap; 

 and boiled 15'. No film was formed in either of them. > 

 was opened on the 30th day; ferment cells and some fila: 

 of a doubtful vegetable appearance were found, xxxi; 

 opened on the 42d day, and contained ferment cells and moiuiu= 

 An escape of gas took place when the flask was opened, x-xx: 

 was opened on the 43d day, and found to contain ferment cells 

 in large numbers, in different stages of cell multiplication ; as m 

 xxxii, there was an escape of gas. 



Exp. XXXIV. (3.) March 27th. Juice of mutton, in a herrae- 

 ticallv sealed flask, was boiled 5' in a Papin's digester, under a 

 pressure of 2 atmospheres. A film formed on the 4th day. B 

 was opened several days later, in the presence of Prof. Gray, and 



