R, H. Chittenden— Oxidation product of Glycogen. 895 



with a carbon residue. The Cranborne graphite requires more 

 Nth the ether than that from the Sevier meteorite 



1 being rubbed up. 



30ut this peculiar substance wilJ be made 

 a little farther on, when I come to speak of the same compound 

 as obtained from the black or carbonaceous - •- 



t:. 



Art. JA.— Contributions frovi the Sheffield Laboratory of Yale 

 College. No. XXXVIIL— Ow the Oxidation product of Gly- 

 cogen with Bromine^ Silver Oxide and Water ; by E. H. Chit- 

 TEi^DEN, Ph.B., Assistant in Physiological Chemistry. 



While submitting an aqueous solution of glycogen to the 

 action of bromine in an open vessel with the aid of heat, it was 

 observed that the strong opacity of the fluid gradually disap- 

 peared, and that, after the removal of the free bromine by partial 

 evaporation, a perfectly clear fluid remained which contained 

 considerable combined bromine. 



This reaction, indicating union between the glycogen and 

 bromine, pointed to the possibility of the formation of an acid 

 from the glycogen by oxidation, in a manner analogous to the 

 formation of "dextronsaure " from dextrin, and " lactonsaure " 

 from lactose, as described by Uabermann,* Barth and Hlasi- 

 s.f The following experiments were undertaken to form, 



or oxidation was as follows : ntty grams ot glycogen dried at 

 100° C, were dissolved in 300 c.c. of distilled water, and this solu- 

 tion transferred to a champagne flask fitted with a caoutchouc 

 stopper, in which was a small stout glass tube drawn out to a 

 point.§ Forty grams of bromine were then added and the stopper 



! flask was then heated in a water bath until tl 



romine had entirely d 

 about two hours' boiling. A heavy 



''-'-' ' ^ ■ " ■ ' ■ ' Dleteiy disappeared by tue 



I up. At the end of this 



first treatment the fluid was perfectly clear and of a pale yellow 



vapors of bromine had entirely disappeared, which required 



color. Afler cooling, the gases were allowed to escape, by 

 breaking the end of the tube in the cork, and, being collected, 

 were found to consist mainly of carbonic acid and bromoforr 

 The stopper was then removed and 40 grams more bromii 



t This Journal, III, vol. x, p.' 26. gAimalen de'r Ch. i p'harm., clii, 316. 



