478 Scientific Intelligence. 



varying from five minutes to five hours and a half, to the boiling 



■«.■ without losing its power of developing life. Two 

 days' exposur. to a temperature oi r>o : Fahr., subsequent to this 

 treatment, sufficed to develop in it swarms of Bacteria. 



The infusion which thus withstood, in one of Dr. Roberts's 

 "plugged bulbs," the temperature of boiling water for 330 min- 

 ute-, v, ;.< completely sterilized in three minutes by boilinu it in a 

 otow neck, and hermeticalh sealing tie !i >k 

 lion. In the ease of the "plugged bulbs" the ob- 

 -istance was due, not to the germs of the infusion, but ro 

 those diffused in the air above it. 



I have also pursued my experiments with closed chambers, from 

 which the floating matter was removed by self-subsidence. With 

 certain new infusions introduced into this inquiry failure after 

 eurred, two or three days g, icraih sufficing to fill the 

 ]''" ! ;1 and pn.t. -i ; ii.piid> with Bacterial life. The vegetable 

 infusions i • ; but a characteristic 



feature of the lite developed in i he last three 



months was the formation in»>n tl,< ir - iri'ae ■< of a thick and .1. dv 

 ;. -uin. Precautions tar greater than those found suc- 

 cessful u year ago failed to protect these infusions from contami- 



i resorted to the mode of calcination by an incandescent plati- 



■pliod with such uniform success in my last inquiry. 

 The wire was brought close to its point of fusion, the period of 

 inc;in escence was doubled, and ext ■ >i " ia - < u was taken to 

 ward off" infection by a ring of cotton-wool. The care proved 

 for, in despite of it. $ .red in the in- 



; afterwards. 



) reproduce the result- with ; 



ase and i 

 ly concentrated by cvap 



<r : they ;l re as clear as distilled water. But in my re- 

 giments, where the care bestowed far exceeded that 

 -ary in m v last inquirv. the animal infusions, like the 

 rthe most oart. into putrefaction. 

 _'•*> ith i . boiled and scaled 



.re (I would empha- c re was no 



- my ol the animal infusions. 





ery possible s 



nation I was able finally to maintain some of the most refractory 

 i. m i losed chambers 



'-''■ ■' v ' '■ : ' . . - : i ' ■ - ' : ' ' • 



It is to be not, q t h; (t , rli.^t ,\p i m i '- u rl i- i e "■> 



m harmony with all the results of the former one, and 

 ; . • -. . ■ ■ ,; . , . . • . 



imer results showed itself in any marked 

 ins f this die a lance? 



: by reference to the 

 " illusions, which were begun early an I ■■ 





