9 
Method.—A small quantity of wheat that had been 
soaked in water for about twelve hours was sealed in a bottle 
containing CO, gas. The method of charging the bottle with 
CO, was os same as that employed in Experiment 4. 
Result.—The grain failed to germinate, but swelled to 
an cede size before i it died. 
Inference.—Germ ating wheat is asphyxiated by CO, 
because of the biecioe of chlorophyll in the plumule. 
EXPERIMENT 8.—To eager ee the effects of an atmo- 
sphere of nitrogen upon wet w 
Method.—The apparatus ead "diethodi employed w 
similar to vum outlined in Experiment 5. In this instanoe 
soaked wheat was used instead of dry grain 
Result.—Germination proceeded Boki. jun sufficient 
to show the development of the radicle and plumule rowth 
Ceased abruptly and the grain died. No chlorophyll was 
formed in the plumule. 
“gps e.—Free oxygen is essential to the development 
germinating seed, and also to the development of 
eee 
SIMPLE METHOD or TESTING AIR-TIGHT BOTTLES. 
Com mpletely immerse the sealed bottle in "€ that is 
a little warmer than the atmosphere. The air within the 
bottle expands, and should the bottle leak, silvery-looking 
air-bubbles will mark the position of the apert 
CoNcLUDING REMARKS. 
- Vermin rae be destroyed in properly-enclosed whea 
stacks by the use of CO, or nitr rogen gas without damage a 
" E aug qualities of the grain. 
M Perfectly ripe, dry/ grain can be safely stored from 
m ES time until the time for seeding in an atmosphere rich 
3. E of CO, gas and nitrogen prevents the develop- 
ment of eari from eggs within a properly-heated stack. 
1 Any ordinary barn properly lined with asbestos sheets 
will = as a suitable storehouse or gas-envelope. dale 
e CO, gas can be generated by pouring dilu 
hinc acid on chips of marble or limestone. r 
The gas should be introduced near the top at one en 
A TUE stack, and the outlet pipe for the displaced air should 
ghey near the bottom of the stack at the other end. 
The escaping air should be tested with a little lime- 
water. When the E AME air causes the lime-water to turn 
