107 
been successfully inoculated, and as only a fleeting infiltration 
the Ss occurred after inoculation, the me 
oned. 
2. Experiments with H. muscae :— 
(a) Larvae placed in the skin of the horse— 
X proboscides of Musca. domestica heavily uper with 
s 
relh were pla an incision in t of mare, and 
emoved five to six hours later Tumefaction of the tissues was 
produced, which increased d the twenty-four hours after 
urin 
inoculation, and then gradually xubelded. About a week later a 
swelling about the size of a Barcelona nut was present. i 
rapidly reduced in size and completely disappeared. 
he day following the first inoculation another inoculation 
was made with the same results as the first. 
ome days later three more inoculations were made. After 
^ rimar mefaction of the tissues had disappeared in from 
wenty-four to forty-eight hours no abnormality could be detected. 
: periments were repeated on the pony all, three 
"sers ons were nly a slight primary tumefaction of 
e tissues resulted which disappeared in ab thirty-six hours, 
; T 
leaving only a very slight thickening, which completely dis- 
Ppeared in from fourteen to twenty-one Gays. 
(b) Larvae added to the conjunctival sac— 
bout the same time as the above experiments were made 
A 
ey were placed in a pipette containing a mixture of equal 
E of mal horse serum nor e on e 
taae were then along with a small quantity of the mix- 
ure to the conjunctival sac (off side) of the mare. e conjunc- 
tiva remained normal in appearance, and no excessive lacrymation 
Was produced 
wever, 
conjunctiva remained normal in appearance. 
(c) Larvae placed on a scarified area of skin— 
the Am eres of the skin of the mare was lightly scdrified, and 
sscides placed on the moist surface, and kept in place 
i 
is experiment was repeated with the same resu. 
IM also repeated on the pony, and no reaction was 
(d) Larvae placed on a moistened area of skin— 
moistened with 
Ls gge on this. The followin 7 th was th 
s : g morning there 
the f tion of an elevation in the skin, but it was not observed 
Same ele evening. is experiment was repeated 
the There was no reaction produced in the tissues to suggest that 
larvae has penetrated the skin. 
