VITALITY OF PUTREFACTIVE OEGANISMS. 197 



Each group embraced two acids and two neutral in- 

 fusions. They were boiled for the following times: — 



1st group 

 2nd „ 

 3rd „ 

 4th „ 



10 minutes. 

 20 „ 

 30 „ 

 60 „ 



After the bulbs had sufficiently cooled, their sealed 

 ends were removed by a file. 



On the 21st of February, less than 24 hours after 

 their preparation, all these bulbs showed signs of yield- 

 ing. On the 22nd they were all turbid, while as 

 regards the comparison of acid and neutral infusions, 

 their condition was this: — 



1st group. 10 minutes. 



3nd group. 20 minutes. 



Srd group. 30 minutes. 



4th group. 60 minutes. . 



' Guildford neutral distinctly more turbid 

 than Guildford acid. Scum on for- 

 mer, none on latter. 

 Old Heathfield neutral not to he dis- 

 tinguished from old Heathfield acid. 

 Both turbid and covered with scum. 

 Much lightened in colour. 

 Guildford neutral distinctly more turbid 



than the acid liquid. 

 Old Heathfield neutral more tm'bid than 

 the acid infusion. 

 / Guildford neutral a little more tm-bid 

 than Guildford acid : diiference small. 

 Old Heathfield neutral more turbid than 

 the acid infusion; the former with 

 scum, the latter none. 

 Guildford neutral distinctly more turbid 

 than Guildford acid ; the former with 

 scum, the latter free from scum. 

 Old Heathfield neutral somewhat more 

 turbid and scummy than the acid 

 liquid : difference not great. 



Here I take it to be absolutely certain that the 

 germs which resisted sterilization were contained in the 



