26 MICROSCOPICAL RESEARCH 



ordinary microtome must be infiltrated with paraffin, and cut by means 

 of some mechanical arrangement. The "Cambridge Rocking Micro- 

 tome" is well suited for this purpose, its traverse movement being 

 actuated to tow o of an inch, and by its use sections have been obtained in 

 my laboratory of animal tissue (suprarenal gland of Cat) cut to two, wVo, 

 and luiuo of an inch in thickness for "test" purposes for Mr. J. E. 

 Storey, of the Manchester Microscopical Society. For ordinary 

 purposes sections seldom require cutting thinner than wiro of an inch 

 in thickness. 



Fig. 1 6. — -The Cambridge 'Rocking" Microtome. 



The primary method of infiltrating vegetable tissue with paraffin is 

 the "Chloroform" or the "Xylol" process, either of which has but little 

 injurious effect on the tissue if properly conducted. I give here full 

 details for the Chloroform method, but Xylol may be substituted for 

 chloroform by those who prefer it: — 



1. Transfer the specimens from 92 per cent, into Absolute alcohol, 



allow to stand twelve to twenty-four hours according to size 

 and density of specimen, change the alcohol once or twice 

 during the time. 



2. Place the specimens in equal parts of Absolute alcohol and 



chloroform, and allow to stand twelve hours. 



