STAINS, REAGENTS, &c. 39 



Chapter III. 



Stains, Reagents, Mountzng-meata, Cements, etc., their Formulce 



and Manipulation. 



The foUowiBg formula and manipulative methods are those daily practised by 

 me in the production of animal and vegetable preparations. The list is a very 

 limited one, but will be found sufficiently extensive to cover the requirements 

 of the general worker. The specialist may extend the formulae and methods to 

 meet his own wants. 



No. I. BORAX CARMINE.— Grenacher. 



Best carmine 5 grams. 



Borax 8 grams. 



Water 480 cc. 



Alcohol 92% 480 cc. 



Dissolve the carmine and the borax in the water by heat. 

 When cold add the alcohol; allow to stand a few days to ripen; filter 

 for use. 



Manipulation. 



1. Pour off the alcohol and cover the sections with the stain, and allow to 

 stand fifteen to thirty minutes. 



2. Pour off the stain and rinse the sections with acidulated alcohol (two 

 drops of Hydrochloric acid to one ounce or 30 cc. of 92% alcohol) ; then rinse 

 with alcohol free from acid. Now allow the sections to stand in alcohol for 

 thirty minutes, changing the alcohol during the interval if necessary. 



3. Pour off the alcohol and cover the sections with oil of cajeput, and allow 

 to stand fifteen minutes to clarify. 



4. Transfer the sections to thin balsam and benzol, and when convenient 

 mount the sections in] balsam. 



