42 MICROSCOPICAL RESEARCH 



Dissolve the alum and the calcium chloride in their respective quantities 

 of water by heat. 



Mix the two solutions, and immediately add 700 cc. of the alcohol slowly ; 

 allow to stand for an hour ; filter. 



Add the hsematoxylin dissolved in the remaining 70 cc. of alcohol, and place 

 the bottle in the sunlight for a few days to ripen ; filter for use. 



Manipulation. 



1. Pour ofi the alcohol and cover the sections with the stain, and allow to 

 stand fifteen to thirty minutes. 



2. Pour off the stain and rinse with accidulated alcohol, then with alcohol 

 free from acid. 



3. Pour off the alcohol and cover the sections with oil of cajeput, and allow 

 to stand fifteen minutes to clarify. 



4. Transfer the sections to thin balsam and benzol. 



5. Mount the sections in balsam. 



No. 4- HiEMATOXYLIN.— Delafield. 



Nuclear Stain fob Vegetable and Animal Tissues. 



200 cc. of a saturated solution of ammonia alum. 



2 grams of hsematoxylin in 12 cc. absolute alcohol. 



Mix the two solutions, and expose to light and air for a week. Filter, and 

 add to the filtrate. 



50 cc. pure glycerine. 



50 cc. of methylic alcohol (wood spirits). 



Allow the solution to stand in the sunlight until the colour develops. Refilter 

 for use. 



Manipulation. 



Sections to be stained from water. 



1. Pour off the water and cover the sections with the stain, allow to stand 

 ten to thirty minutes according to requirements, or nature of the tissue under 

 treatment. 



