STAINS, REAGENTS, &c. 43 



2. Pour off the stain, and rinse the sections in two or three changes of 25% 

 alcohol, then graduate to 92% alcohol. 



If the sections are overstained, or if the staining be too diffuse, treat them 

 with the acidulated alcohol until the stain is removed from all parts except the 

 nucleus. The staining may be made more precise in its action if the stain be 

 diluted with several times its own volume of water, and the time in staining 

 be prolonged accordingly. 



3. Clear the sections, if uncelloidinized, in oil of cajeput. 



4. Transfer the sections to thin balsam and benzol, and when convenient, 



5. Mount the sections in balsam. 



No. 5. AMMONIATED HiEMATOXYLIN.— Ehrlich. 



Nuclear Stain fob Vegetable and Animal Tissues. 



Hsematoxylin 15 grams. 



Am.monium carbonate 15 grams. 



Absolute alcohol 300 cc. 



Place in a large bottle and shake occasionally for three days, leaving the 

 stopper out during the intervals. 



Place the solution in an open dish, and allow it to evaporate to dryness at 

 the temperature of the air. 



Dissolve the crystalline product obtained in 



Alcohol 92% 750 cc. 



Pure glycerine 750 cc. 



Pure water 750 cc. 



Glacial acetic acid 75 cc. 



Ammonia alum 15 grams. 



Filter for use. 



Manipulation. 

 Sections to be stained from 25% alcohol. 



1. Pour off the alcohol and cover the sections with the stain, allow to stand 

 fifteen to thirty minutes. 



