44 MICROSCOPICAL RESEARCH 



2. Pour off the stain and rinse the sections in two or three changes of 25% 

 alcohol, and graduate to 92%. Reduce the colour if necessary with acidulated 

 alcohol. 



3. Pour off the alcohol and cover the sections, if uncelloidinized, with oil of 

 cajeput; allow to stand ten to fifteen minutes to clarify. 



4. Transfer the sections to thin balsam and benzol, and when convenient 



5. Mount the sections in balsam. 



No. 6. BRAZILIN. 



NucLEAE, Stain for Animal and Vegetable Tissues. 



A. The mordant. 



Iron Alum 1 gram. 



Alcohol 75% ... 100 cc. 



Dissolve and filter for use. 



B. The stain. 



Brazalian crystals 1 gram. 



Alcohol 75% 100 cc. 



Dissolve and filter for use. 



Manipulation. 



1. Pour off the alcohol and cover the sections with the mordant, A; allow to 

 stand for an hour. 



2. Pour off the mordant and rinse once with 75% alcohol. 



3. Pour off the alcohol and cover the sections with the stain, B ; and allow 

 to stand three, six or more hours, according to nature of tissue dealt with. The 

 thinner the section the longer it should remain in the stain. 



4. Pour off the stain and rinse the sections in several changes of 92% alcohol. 

 Reduce the colour if necessary with acidulated alcohol. 



5. Clear the sections, if uncelloidinized, with oil of cajeput. 



6. Transfer the sections to thin balsam and benzol, and when convenient 



7. Mount the sections in balsam. 



