STAINS, REAGENTS, &c. 45 



No. 7. PICRO-CARMINATE OF AMMONIA. 



A 

 Picric acid crystals 9 grains in 400 cc. of 92% alcohol. 

 Best carmine 2 grams in 40 cc. of liquid ammonia. 



Dissolve the two mixtures, and then add to the carmine solution, 400 cc. 

 of water, and then add to this the picric acid solution. Filter for use. 



B 



A saturated solution of ammonium picrate in 92% alcohol. 



Manipulation. 



1. Pour off the alcohol and cover the sections with A, and allow to stand 

 one hour. 



2. Pour off A, and rinse the sections quickly with alcohol. 



3. Pour off the alcohol and cover the sections with B, and allow to stand 

 fifteen minutes. 



4. Pour off B, and rinse the sections two or three times with alcohol. 



5. Pour off the alcohol and cover the sections with oil of cajeput to clarify. 



6. Transfer the sections to thin balsam and benzol. 



7. Mount the sections in balsam. 



No. 8. ANILINE PICRATE AND GOSSYPIMINE. 



Foii. Double Staining, Yegetable, and Animal Tissues. 

 Sections to be stained from water. 



A 



Aniline blue crystals 3 grams. 



Picric acid crystals 2 grams. 



Water 960 cc. 



Dissolve the aniline blue crystals in the water, then add the picric acid ; 

 filter for use. 



