46 MICROSCOPICAL RESEARCH 



B 



Gossypimine crystals 2 grains. 



Water 900 cc. 



Alcohol 92% 60 cc. 



Dissolve and filter for use. 



Manipulation. 



1. Pour off the water and cover the sections with A, and allow to stand thirty 

 minutes. 



2. Pour off A, and rinse the sections in two or three changes of 25% alcohol, 

 using a trace of acid to reduce the colour if necessary. 



3. Pour off the alcohol and cover the sections with B, and allow to stand 

 thirty minutes. 



4. Pour off B, and rinse the sections quickly with 75%, finishing off with 

 92% alcohol. 



5. Clear the sections, if uncelloidinized, in oil of cajeput. 



6. Transfer the sections to thin balsam and benzol. 



7. Mount the sections in balsam. 



In working with aniline blue and gossypimine, it may be found advisable to 

 reverse the order of manipulation by staining first with B; this will be found 

 to be the case when the tissues dealt with are fully formed or highly lignified, 

 i.e., that stain must be used first for which the tissues have the greatest affinity, 

 and retains it the most persistently. In the knowledge, therefore, of the nature 

 of the tissues dealt with lies the secret of all good staining. 



No. 9. HiEMATOXYLIN AND GOSSYPIMINE. 



To double stain sections with hsematoxylin and gossypimine, substitute 

 haDmatoxylin for aniline picrate and manipulate as for No. 8, using acidulated 

 alcohol to reduce the hsematoxylin colour if necessary. 



