STAINS, REAGENTS, &c. 47 



No. 10. ANILINE BLUE. 



A Single Stain foe Vegetable Tissue. 



Aniline blue crystals 1 gram. 



Alcohol 92% 300 cc. 



Dissolve and filter for use. 



Manipulation. 



1. Pour off the alcohol and cover the sections with the stain; allow to stand 

 thirty minutes. 



2. Pour off the stain and rinse the sections in two or three changes of alcohol; 

 using the acidulated alcohol to reduce the colour if necessary. 



3. Clear the sections, if uncelloidinized, in oil of cajeput. 



4. Transfer the sections to thin halsam and benzol, and when convenient 



5. Mount the sections in balsam. 



No. II. MALACHITE GREEN.— A Counter Stain. 



Malachite green 1 gram. 



Alcohol 92% 300 cc. 



Dissolve and filter for use. 



Manipulation. 



1. After staining with carmine, formula No. 1, 



2. Pour off the alcohol and cover the sections with the malachite green stain, 

 and allow to stand fifteen to thirty minutes. 



3. Pour off the stain and rinse the sections quicJdy in two or three changes 

 of alcohol; and as soon as the carmine stain stands out clearly pour off the 

 alcohol and 



4. Cover the sections with oil of cajeput to clarify. 



5. Transfer the sections to thin balsam and benzol, and when convenient 



6. Mount the sections in balsam. 



