48 MICROSCOPICAL RESEARCH 



No, 12. SAFRANIN.— A Counter Stain. 



Saf ranin 3 grains. 



Water 430 cc. 



Alcohol 92% 540 cc. 



Dissolve by lieat, when cold filter for use. 



Manipulation. 



1. After staining with hsematoxylin formula No. 3, 



2. Pour off the alcohol and cover the sections with safranin, and allow to 

 stand thirty minutes. 



3. Pour off the stain and rinse the sections quicldy in two or three changes 

 of alcohol, and as soon as the hsematoxylin colour stands out clearly, pour off 

 the alcohol and 



4. Cover the sections with oil of cajeput to clarify. 



5. Transfer the sections to thin balsam and benzol, and, when convenient, 



6. Mount the sections in balsam. 



No. 13. EOS IN.— A Counter Stain. 



Eosin 1 gram. 



Alcohol 92% 300 cc. 



Dissolve and filter for use. 



Eosin may be employed as a counterstain with either aniline blue or 

 hsematoxylin, the manipulation being exactly the same as for Nos. 11 and 12. 



MOUNTING MEDIA, etc. 



Canada Balsam. 



Canada balsam is supplied commercially in its crude condition containing 

 essential oils and similar substances, which must be eliminated by evaporation 

 before the balsam can be used for microscopical purposes. 



