50 MICROSCOPICAL RESEARCH 



Carbolized Water. 



Pour off the clear liquid from A, and to this add B, and allow to stand for 

 an hour to settle; filter for use. 



Sections must always be placed in water before they are bleached. 



Manipulation. 



1. Pour off the water and cover the sections with the bleaching solution, 

 allow to stand five to thirty minutes, or until the sections are bleached white. 



2. Rinse the sections in several changes of water, or allow them to soak until 

 quite free from the lime solution. 



3. Transfer, or graduate if necessary, the sections to alcohol, ready for 

 staining. 



The washing process may be facilitated by the addition of a few drops of 

 hydrochloric acid to the water. 



