TYPE PREPARATIONS 



53 



Root of Indian Coun, 'Zea maize,' ^/i^ of an incli in diameter. 



Fixed in 92% alcohol. 

 Cut transverse sections ^/^oo of an incL. in thickness. Place the sections 



in alcohol. 



Bleach the sections. 



Manipulate Nos. 1 and 11. 



Compare the preparation with Fig. 4. Plate I. 



A. B. 



Fig. 26. — Germinating box. A. 3, wood box, with strips of wood laid across the open 

 top, over which a strip of flannel is let into folds. 1, loose ends of flannel leading 

 to vessels containing water, which feeds the seed by osmosis. 2, grains of barley 

 in the folds of the flannel. B. The seeds after being germinating for 

 6 days. 



Developing Radicles of Barley. 



Place some grains of barley in the germinating box. Fig. 26, allow them to 

 germinate for twelve hours (the flannel must be damp when the grains are 

 placed in). Now take out the grains, and with a sharp section knife cut them 

 in half transversely and place the germinal half direct into the chromo-acetic 

 solution, and allow them to ' fix ' for twenty-four hours ; now wash and graduate 

 to alcohol as directed (see collecting and fixing of tissues). 



Celloidinize the speciviens. 



Cut transverse sections Visoo of an inch in thickness through the radicle end 



of the germ ; place the sections in alcohol. 



Manipulate No. 6. 

 Compare the preparation with Fig. 5. Plate II. 



