56 MICROSCOPICAL RESEARCH 



Stem of Lime-tree, 'Tilia europoea.' Fixed with 92% alcohol. 



a. Cut transverse sections ^/75o of an inch in thickness. Place the sections in 



alcohol. 



b. Cut tangential longitudinal sections ^/laoo of an inch in thickness, passing 



through the phloem. Place in alcohol. 



c. Cut t.angential longitudinal sections -^/looo of an inch in thickness, passing 



through the xylem. Place in alcohol. 



Bleach the three series of sections. 

 Manipulation for a Nos. 1 and 11; for b and c, Nos. 3 and 12. 



Mount all the sections on one slide, as in the order named. 

 Compare the preparation with Figs. 15, 16 and 17. Plate V. 



Stem of Drac^na, ' Cordyline rubra.' Fixed with 92% alcohol. 

 Cut transverse sections ^/eoo of an inch in thickness. Place the sections in 



alcohol. 



Bleach the sections. 



Manipulate Nos. 1 and 11. 



Compare the preparation with Fig. 18. Plate V. 



Stem of Pine-tree, 'Pinus sylvestris.' Fixed with chromo-acetic solution. 

 For development and formation of parts. 



a. Cut longitudinal-median sections through apex of young stem, ^/jooo of an 



inch in thickness. 



b. Cut transverse sections i/„o of an inch in thickness, half -inch below growing 



point. 



c. Cut transverse sections i/„o of an inch in thickness, two inches below growing 



point. 



d. Cut radial longitudinal sections i/„o of an inch in thickness, two inches below 



growing point. 



Place each group of sections in alcohol, as cut. 



Manipulation for a, No. 6. Manipulation for b, c, d, No. 9. 



Compare the preparations with Figs. 19, 20, 21, 22 Plate VI. 



Also Fig. 23. Plate VII. 



