TYPE PREPARATIONS 6i 



c. Boil over the bunsen flame for two or three minutes. 



d. Pour the contents of the test-tube into cold water, shake well for two or 



three minutes. 



e. Pick out the separated cuticles and place them in clean water to wash for an 



hour or two. 



If the intermediate tissue has not been completely removed, the 

 boiling process must be repeated, at the same time great care must be 

 taken not to carry the boiling beyond this point, or the guard cells of 

 the stomata will also be removed. After washing the cuticles 

 /. Transfer to 92% alcohol. 



Manipulate No. 6. 

 Compare the preparation with Fig. 42. Plate XII. 



Stomata in Gheen Stem, Shee Oak, 'Casuarina equisetifolia.' Developing twig 



^/lein. diameter. .Fixed with chromo-acetic solution. 



Cut transverse sections ^/i2oo of ^^ inch in thickness. Place the sections in 



alcohol. 



Manipulate Nos. 1 and 11. 



Compare the preparation with Fig. 43. Plate XII. 



Sting of Nettle, ' Urtica dioica.' Fixed with the chromo-acetic solution. 

 "With a sharp scalpal remove longitudinal strips of cuticle, with its stings, 

 from the midrib of the leaf, divide the strips as required and place in alcohol. 



Manipulate Nos. 1 and 11. 

 Compare the preparation with Fig. 44. Plate XII. 



Phyllodium of Acacia, 'Acacia decurrens.' Fixed with chromo-acetic solution. 

 Cut transverse sections ^ j 2000 of ^^ inch in thickness. Place the sections 



in alcohol. 



Manipulate No. 8. 



Compare the preparation with Fig. 45. Plate XIII. 



