TYPE PREPARATIONS 65 



Place the sections in alcohol. 



Manipulate No. 8. 



Compare the preparation with Fig. 59. Plate XVI. 



Entire Flower of the Wheat, 'Triticum vulgaris.' 



Fixed with the chromo-acetic solution. 

 Specimen collected before emerging from the sheath, and dissected out. 



Manipulate No. 1. 

 Compare the preparation with Fig. 60. Plate XVII. 



Stigma of Wheat, ' Triticum vulgaris.' Fixed with chromo-acetic solution. 

 Specimen dissected out of its sheath about ten days prior to its natural time 



of opening. 

 Manipulate No. 1. 

 a. Place the stigma in equal parts of alcohol, glycerine, and water. 

 h. Place the stigma in two parts pure glycerine, and one part each of alcohol 

 and water. 



c. Place the stigma in pure glycerine. 



Allow to stand in each solution of a and h for an hour. 



d. Mount the specimen in glycerine jelly. 



Compare the preparation with Figs. 61 — 62. Plate XVII. 



CELL-STRUCTURE AND CELL-CONTENTS. 



Place some grains of wheat, ' Triticum vulgaris,' in the germinating box, 

 and allow them to germinate for sixteen hours. Fix with the chromo-acetic 

 solution, and then graduate to 92% alcohol. (See Fig. 26). 

 Cut longitudinal-median sections of entire seed, ^/eoo of an inch in thickness. 



Place the sections in alcohol. 



Manipulate No. 1. 



Compare the preparation with Fig. 63. Plate XVIII. 



