TYPE PREPARATIONS 67 



Embryo of Wheat, collected before fertalization. 



Fixed with chromo-acetic solution. 

 Cut longitudinal sections ^ j -,50 of an inch in thickness. Place the sections in 



alcohol. 



Manipulate No. 6. 



Compare the preparation with Fig. 68. Plate XIX. 



Embryo of Wheat, collected after fertilization. 



Fixed with chromo-acetic solution. 

 Cut longitudinal sections ^ j rso oi an inch in thickness. Place the sections in 



alcohol. 



Manipulate No. 6. 



Compare the preparation with Fig. 69. Plate XIX. 



Wheat-rust, 'Puccinia graminis.' Uredospores, on leaf of thistle. 



Fixed with 92% alcohol. 

 Celloidinize the specimen. 

 Cut transverse sections of leaf ^/looo of ^n inch in thickness. Place the sections 



in alcohol. 



Manipulate No. 10. 



Compare the preparation with Fig. 70. Plate XX. 



Wheat-eust, ' Puccinia graminis.' Teleutospores on stem of wheat. 



Fixed with 92% alcohol. 

 Celloidinize the specimen. 

 Cut transverse sections of the stem V1500 of an inch in thickness. Place the 



sections in alcohol. 



Mount without staining. 



Compare the preparation with Fig. 71. Plate XX. 



