TYPE PREPARATIONS 69 



Feen, ttallus of ' Gymnogramina sp.' Fixed witli clironio-acetic solution. 



Infiltrate the specimen with paraffin. 



Cut vertical sections •'/sooo of an inch in thickness, with the mechanical 



microtome. 



Mount the preparation as described for the paraffin method. 



Manipulate No. 6. 



Compare the preparation with Fig. 77. Plate XXI. 



Staech in stem of tradescantia, ' Tradescantia discolor.' 



Fixed with chromo-acetic solution. 

 Cut transverse sections of stem ^/^oo of an inch in thickness. Place the sections 



in alcohol. 



Manipulate No. 3 and Gossypimine (8B). 



Compare the preparation with Fig. 78. Plate XXII. 



Staech in stem of pellionia, 'Pellionia Daveauna.' 



Fixed with chromo-acetic solution. 

 Cut transverse sections of stem 1/400 of an inch in thickness. Place the sections 



in alcohol. 



Manipulate No. 3 and Gossypimine (8B). 



Compare the preparation with Fig. 79. Plate XXII. 



Apical-cell in root of fern, 'Aspidium Filix-mas.' 



Fixed with chromo-acetic solution. 



Apex of developing roots collected about the middle of May. 



Infiltrate the specimens with paraffin. 



Cut longitudinal median sections i/eooo of an inch in thickness. 



Mount the sections as described for the paraffin method. 



Manipulate No. 6. 



Compare the preparation with Fig. 80. Plate XXII. 



