88 MICROSCOPICAL RESEARCH 



PLATE IX. 



STEM STRUCTURE. 



EiG. 31. Transverse section immediately below vegetative bud of 

 ' Equisetum telmateia ' x 75 dia. Tissue fixed in Cbromo- 

 Acetic solution. Stained with Borax Carmine and Malachite 

 Green. 1. Nucleated cortical tissue. 2. Bast. 3. Isolated 

 annular vessels in intercellular space. 4. Primary nucleated 

 tissue. 



Fig. 32. Transverse section through vegetative bud of 'Equisetum 

 telmateia ' x 10 dia. Fixed as above. Stained Carmine. 



1. Central axis of stem. 2. Zone of vascular bundles. 

 3 and 4. Leai-sheaths in various stages of development, the 

 outer layer dividing into separate leaves. 



Fig. 33. Longitudinal median section of an older vegetative bud 

 than above x 30 dia. Fixed as above. Stained with Borax 

 Carmine and Malachite Green. 1. Central ground tissue. 



2. Nodal diaphragm. 3. Secretion cells. 4. Vascular 

 system. 5. Lateral bud. 6. Cavity of vascular bundle. 

 7. Growing point of lateral bud. 8. Caniculi in formation. 



Fig. 34. Longitudinal median section through growing point of 

 lateral bud in Fig. 33 (7). 1. Meristem tissue. 2. Bud 

 sheath. 3. Apical cell. 4. Zone of dividing nucleated 

 tissue. Cut ^/ 1000 ill- Stained Brazilin x 120 dia. 



