ACTION OF LIGHT ON MICRO-ORGANISMS 379 



The flasks so treated were placed in an incubator at 

 37° C, and the following further experiments made 

 with them : — 



Animal Experiment. ^On October 22, 1892, 0*5 c.c. 

 of the water (to which broth had been added on 

 October 15, 1892) in the flask ' 1 I, Thames water, 

 unfiltered, and infected with anthrax on March 18, 

 1892, exposed to daylight since April 9, 1892,' was 

 .subcutaneously injected into a white mouse. The 

 mouse died within 2 days 18 hours. The body ex- 

 hibited extensive oedema and the spleen was much 

 enlarged ; the latter was found fall of anthrax bacilli, 

 the presence of which was confirmed by cultivation in 

 gelatine. 



Animal Experiment. — On October 18, 1892, 0'5 c.c. 

 of the water (to which broth had been added on 

 'October 15, 1892) in the flask '• 5 I, Thames water, 

 paper-filtered, and infected with anthrax March 18, 

 1892, exposed to daylight since April 9, 1892,' was 

 .subcutaneously injected into a white mouse. The 

 mouse died within 1 day 1 9 hours. Only few bacilli 

 were found in the spleen, but more in the kidney ; 

 their presence was confirmed by gelatine cultivations 

 from both organs. 



These experiments show, then, that in the flasks in 

 question [unsterilised Thames water exposed for upwards 

 of six months to diffused daylight)^ although the number of 

 -anthrax germs had been so far reduced that 1 c.c. would 

 not kill mice^ yet after nourishme7it with broth they were so 

 revivified as to be fatal to these animals when injected in 

 the same or even a smaller quantity. 



