SPIRILLA 401 



VIBEIO AQUATILIS 



LIQUEFIES GELATINE 



Authority. — Giiuther, ' Ueber eine neue ira Wasser gefunclene Komma-bacil- 

 lenart,' Deutsche medicmischeWochenschrift, No. 49, 8 December, 1892, p. 1124. 

 See also Kieszling, ' Ein dem Choleravibrio ahnlicher Komma-bacillus,' Arheiten 

 a. d. kaiserlichen Gesundheitsamte, voL viii., 1893, p. 430. 



Where Found. — Found in river Spree water at Stralau at the intake of the 

 Berlin waterworks. Found by Kieszling in slimy water which had been used 

 for washing the sand used in the water-works at Altona, 



Microscopic Appearance. — Eesembles in every particular Koch's Comma 

 bacillus (see p. 399). Possesses one cilium at one pole. 



Cultures. — 



Gelatine Plates.— Forms circular and perfectly smooth -rimmed colonies, 

 brown in colour, and having very fine granular contents, and therefore differs 

 from Koch's Comma bacillus. If near other liquefying colonies on a gelatine 

 plate their edge becomes less sharply defined. 



Gelatine Tubes, — Grows exclusively on the surface; the basin-shaped 

 liquid depression, however, gradually extends downwards, but in the needle's 

 path in the depth below the liquid depression practically no growth makes its 

 appearance. 



Agar-agar. — Grows like Koch's Comma bacillus both at 15°to 20° C. and at 

 37° C. 



Potatoes. — When tried on four different kinds of potatoes no growth took 

 place, whether at 15° to 20° C, or at 37° C. 



Broth. — Refuses to grow in alkaline and neutral broth at 37° C. At 

 21° to 22° C. hardly any growth takes place, only after some weeks a very slight 

 trace of turbidity made its appearance in both descriptions of broth, which was 

 traced to the growth of the vibrio. 



Kemarks, — Does not exhibit the ' cholera-red ' or nitrosoindol reaction, and is not 

 pathogenic to animals. 



BACILLUS CHOLEROIDES a 



I LIQUEFIES GELATINE | 



Authority. — Bujwid, 'Ueber zwei neue Arten von Spirillen in Wasser,' 

 Centralhlatt f. Bakteriologie, vol. xiii., 1893, p. 120. 



Where Found. — In river water. 



Microscopic Appearance.— Resembles Koch's Conuna sxnrillum ; the move- 

 ments are, however, not so rapid. 



Cultures. — 



Gelatine Plates. —Eesembles the Comma spirillum when grown at 10° to 

 12° K., but at a higher temperature the colonies are broader and more superficial, 

 and do not sink so deeply into the gelatine, which becomes gi-adually turbid. 

 Under a low power the contour is more regular than is the case with the Comma 

 spirillum ; they are almost smooth or very finely granular. Does not give rise 

 to an odour of indol, but recalls that of methyl-mercaptan. 



Gelatine Tubes.— Grows on the surface, liquefying only the upper layers. 

 At about 10° to 12° E. the gelatine is more slowly liquefied, and the well-known 

 air-bubble appearance is produced. It hardly grows at all in the depth. 



Agar-agab. — Grows luxuriantly in the incubator and gives rise to an odour 

 resembling methyl-mercaptan. 



Broth. — Eenders it slightly turbid, and no pellicle is produced. 



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