428 MICRO-ORGANISMS IN WATER 



BACILLUS TEEMO 



LIQUEFIES GELATINE 



Authority. — Mae6, TraiU pratiqiie de BacUriologie, 1892. 



"Where Found.— According to Mac6, there exists a true Bacillus termo iii 

 water, whilst the Bacterium tertno so often referred to by earlier writers pro- 

 bably includes a considerable number of different varieties, such as Bacillus 

 fluorescens Uquefaciens, Proteus vulgaris, &c. (Boux, loc. cit, p-. 331.) 



Microscopic Appearance. — Thick rods, about 1-4 p. long and 0-8 u, broad, 

 usually in pairs, sometimes in chains. It is very active and is possessed of 

 flagella. 



Cultures. — 



Gelatine Plates. — In eight hours a small whitish colony with a greyish peri- 

 phery is visible, surrounded by a zone of liquid gelatine. At the end of three 

 or four days the centre is opaque, surrounded by a liquid 2 to 4 mm. in diameter. 

 The periphery is pale and transparent, very sinuous, and sometimes lobular, 

 exhibiting at 20° C. movements of the liquid which appear to displace the 

 lobular projections, causing the whole colony to produce the illusory impression 

 of an amceba. Sometimes the gelatine assumes a greenish tint in the vicinity 

 of the colony. 



Gelatine Tubes.— Forms a cup-shaped depression which becomes elongated 

 into a funnel. The liquefaction soon reaches the walls of the tube. 



Beoth. — Benders the liquid turbid, and forms a light and brittle pellicle and 

 only a slight deposit. 



Kemarks. — It is strictly aerobic. Is a powerful agent in the decomposition of 

 animal and vegetable matters. 



BACILLUS OF MOUSE SEPTICEMIA (Koch) 



(Bacillus murise^ticus) 



LIQUEFIES GELATINE 



Authority. — Gaffky, ' Ueber die Aetiologie der Wundinfektionskrank- 

 heiten,' Mitth. a, d. kaiserlichen Gesundheitsamte, vol. i., 1881, p. 80 ; Loeffler, 

 loc. cit., p. 135. 



Where Found. — In the river Panke ; also by Bintaro Mori {Zeitschrift 

 f. Hygiene, vol. iv., 1888) in drain-water. 



Microscopic Appearance. — Very small bacillus, 0-8 to 1-0 ju long and O'l to 

 0-2 jit broad; occurs frequently in pairs. It is not motile. Forms spores. Is 

 stained by Gram's method. 



Cultures.— 



Gelatine Plates. — Does not grow on ihe surface ; forms ill-defined colonies 

 resembling small whitish clouds. Above each colony a small shallow depression 

 is formed in the gelatine. 



Gelatine Tubes. — Grows slowly, producing a delicate white diffused cloudy 

 appearance. In strongly alkaline gelatine liquefaction sometimes takes place. 



Agab-Aqar. — Forms restricted yellowish white colonies. 



Potatoes. — No growth. 



Remarks. — Pathogenic to house mice ; the latter, on being eubcutaneously inocu- 

 lated, die in from forty to sixty hours. Field-mice are immune. 



