BACILLI 431 



BACILLUS HYDltOPHlLUS FUSCUS 



LIQUEFIES GELATINE 



Authority. — Sanarelli, * Ueber einen neuen Mikro organ ismus des Wassers,' 

 Centralblatt f. Bakteriologie, vol. ix., 1891, pp. 193 and 222. 



Where Found. — In well water supplied to the University of Siena. 



Microscopic Appearance.— When taken from gelatine cultures the bacilli 

 vary in size from 2 to 3 /a up to from 12 to 20 fj. long, forming wavy threads, whilst 

 some are so short that they have an egg-shaped or even ball-like appearance. 

 Differences are also observable in their width. On agar-agar they are much more 

 constant in form and mostly from 1 to 3 ,ti long, the smaller individuals are egg- 

 shaped. No spore formation observed. It is very motile. 



Cultures. — 



Gelatine Plates. — Visible in from eighteen to twenty-four hours as circular 

 and regular colonies with a smooth surface. They are greyish white in colour, 

 but in direct light a faint bluish refraction is visible in their vicinity. The gelatine 

 is so rapidly liquefied that their further progress cannot be watched. 



Gelatine Tubes. — After twelve hours the gelatine is liquefied along the whole 

 extent of the needle's path ; the contents of the canal are turbid and contain 

 white flocculent material. In three to four days the whole of the gelatine is fluid, 

 and a thick whitish flocculent deposit is visible. 



Glycerine- AG AE. — At 37° C, a few hours after inoculation a light blue 

 diffused fluorescence is visible on the surface, after which an abundant and ex- 

 tensive growth appears and the condensed water becomes turbid. In from 

 twenty-four to thirty-six hours large bubbles of gas appear in the depth. Later 

 on the blue fluorescence disappears and the growth becomes thicker, and from 

 a dirty grey it becomes brownish in colour. 



Blood Serum. — The serum is rapidly liquefied all along the needle's path, 

 already after twelve hours a fairly deep furrow being visible. 



Potatoes. — After twelve hours along the needle's path a fine straw yellow 

 pellicle is formed, which gradually becomes yellow, and in four to five days has 

 turned so brown that it resembles exactly the growth of the Glanders bacillus 

 on this medium. 



Bboth. — The liquid is turbid in twelve hours, and later a thin pellicle forms 

 on the surface. 



Bemarks. — Pathogenic to frogs, toads, salamanders, fresh-water eels ; also to 

 waffm-blooded animals, such as white mice, bats, guinea-pigs, newly-born kittens, 

 dogs, and rabbits ; also pigeons and fowls. 



BACILLUS FUSCUS 



Authority. — Zimmermann, Die Bahterien uiiserer Trink- unci Nutzwdsser, 

 insbesondere des Wassers der Chevmitzer IVasserleitung, Chemnitz, 1890. 



Where Found. — In water. Found also in water by Migula (Zcitschrift 

 f. Hygiene, vol. viii., 1890, p. 357). 



Microscopic Appearance. — Very variable in size ; longer or shorter, straight 

 and distinctly bent rods, with rounded ends and irregular contour ; about 0'63 fi 

 broad. It is not motile. No spore formation observed. 



Cultures. — 



Gelatine Plates, — In the depth small yellow brown dots, but on the surface 

 raised pin-heads irregular and uneven. Under a low power the depth colonies 

 are sharply defined and more or less circular, but frequently with an irregular 

 and indented edge, resembling granular discs, and greyish or brownish yellow 

 in colour. The surface colonies are brownish yellow in the centre, and are 

 surrounded by a highly refracting shining border. The gelatine is not 

 liquefied. 



Gelatine Tubes. — Forms projecting butt on -growth, which later expands and 

 becomes of a chrome-yellow colour. 



Agar-agab. — Similar as in gelatine tubes. 



Potatoes. — Forms a dark chrome-yellow crumbling expansion. 



