440 MICRO-ORGANISMS IN WATER 



BACILLUS EUBIDUS 



I LIQUEFIES GEL ATINE | 



Authority. — Eisenberg, BaJcteriologische Diagnostik, 1891, p. 88. 



"Where Found.— In water. 



Microscopic Appearance.— Meclium-sized bacilli with rounded ends, often 

 forming long threads. It is very motile. 



Cultures.— 



Gelatine Plates. — Round finely granular liquefying colonies, with a 

 smooth rim and coloured centre. 



Gelatine Tubes. — Liquefies the gelatine, slowly producing a brownish red 

 pigment. 



Agar-agae. — Forms a rapidly spreading brownish red expansion. 



Potatoes. — Produces a fine brownish red-coloured growth, which is not 

 restricted to the point of inoculation. 



Blood Sekum. — Liquefies the serum, producing a red pigment. 



Bemarks, — It will not grow at higher temperatures. 



BACILLUS PEODIGIOSUS 



LIQUEFIES GELATINE 



Authority. — Ehrenberg. See also ' XJeber einige durch Bakterien gebildete 

 Pigmente,' Schroeter, Beitrdge zur Biologic der Pflanzen, Heft ii., 2nd 

 edition, Breslau, 1881, p. 109. See also, ' Studies on some new Microorganisms 

 obtained from Air,' Percy and G. C. Frankland, Phil. Trans., vol. clxxviii., 1887, 

 p. 284. 



Where Found. — On moist bread, potatoes, etc. Found in water by Tils 

 (loc. cit.) and Percy Frankland {loc, cit). 



Microscopic Appearance, — The cells are rather longer than broad, the 

 largest forms being about 1'7 fx in length and about 1 fi in width ; they are 

 frequently found hanging together in pairs. It is not motile. (See Plate I. In.) 



Cultures. — 



Gelatine Plates. — After two days the colonies are visible to the naked eye 

 as circular depressions, each having a red centre. Under a low power the less 

 developed colonies in the depth are devoid of red colour, are finely granular, 

 and have a very irregular contour. The surface colonies, on the other hand, 

 have a distinctly red nucleus, surrounded by a very thin and finely granular 

 brownish growth, having a very irregular contour. (Percy Frankland.) (See 

 Plate I. iB., Ic.) 



Gelatine Tubes. — Grows very rapidly, liquefying the gelatine in the form 

 of a conical sack, which soon extends across the tube at the top, and, gradually 

 passing downwards, involves the whole tube. The liquid is very turbid, with 

 an abundant flocculent deposit of an intensely crimson colour. Near the surface 

 there is generally seen adhering to the glass a thin layer of still darker red- 

 colouring matter, which has the peculiar fluorescence of an aniline colour when 

 in a concentrated state. (Percy Frankland.) (See Plate I. 1a.) 



Agar-agar. — Grows very rapidly, producing a deep, blood-red, smooth and 

 shining expansion, the colour being restricted to the surface. 



Blood Serum.^As on agar, only the serum becomes liquefied-. 



Potatoes. - Grows luxuriantly, producing a magnificent red pigment, which 

 has a metallic brilliancy upon it. 



Broth.— Renders the broth turbid, and produces at first a white deposit, 

 which later becomes pinkish. 



Kemarks. — By long-continued culture it often loses its x^ower of pigment-produc- 

 tion, which may generally be restored by cultivation on potatoes. 



