BACILLI 465 



BACILLUS INCANU8 



LIQUEFIES GELATINE 



Authority.— Pohl, ' Ueber Kultur unci Eigenschaften einiger Sumpfwasser- 

 bacillen,' Centralblatt f. Bakteriologie, vol. xi., 1892, p, 192. 



"Where Found.— In marsh-water. 



Microscopic Appearance. — Bacilli 1*7 /i long and 0-4 m broad. Occurs 

 usually lying parallel, two to four individual bacilli side by side. It is slightly 

 motile. 



Cultures.— 



Gelatine Plates. — Forms circular granular colonies with a smooth dark 

 rim. The gelatine is only slightly liquefied. 



Gelatine Tubes. — Produces a grey white raised expansion on the surface, 

 and grows visibly in the depth. Slight liquefaction takes place after forty-eight 

 hours, which only progresses very slowly. 



Agae-agae.— Forms grey white granular growths all along the needle's path. 

 The condensed water remains clear. 



Potatoes. — Produces an abundant and spreading grey thread-Uke growth. 



Semarks. — "When cultivated on gelatine tinted blue with litmus, the colour of 

 the latter remains unaltered, and therefore no acid is produced. 



BACILLUS IRIDESCENS 



LIQUEFIES GELATINE 



Authority. — Tatarofi, Die Dorpater Wasserbacterien, Dorpat, 1891, p. 57- 



"Where Found. — In Dorpat water. 



Microscopic Appearance. — Bacillus varying in length from 3*5 to 5-2 fi ; 

 forms also long (8-7 to 10*5 ^u) bent threads. Only capable of slight oscillatory 

 movements. Forms spores. 



Cultures. — 



Gelatine Plates. — Forms irregular blue, greenish yellow iridescent colonies, 

 consisting of flakes composed of numerous tiny shining plates or scales ar- 

 ranged like slates on a roof. After a time a yellow central disc is visible, from 

 which liquefaction starts. Under a low power the depth colonies are granular, 

 shining yellow and circular discs. The surface colonies exhibit at first a pale 

 yellow shining, ill-defined disc, which later becomes broken up into isolated 

 windings, and surrounded by a radially folded outer zone, in which isolated con- 

 centric foldings also become visible. The appearance of the colony is very cha- 

 racteristic, and may be compared to the convolutions of the brain; the 

 central windings are fine and sharply defined, and shining yellow, whilst 

 towards the periphery they are coarser, not so sharply defined, and white. 



Gelatine Tubes. — Forms a thread-like growth in the depth, and on the sur- 

 face a delicate shining basin, the centre of which is yellowish and depressed, 

 whilst the outer zone is broad, irregular and iridescent. In three to four days- 

 liquefaction commences in the centre, and a slimy yellow mass collects in the 

 depression. 



Agar-agar and Glyceeine-agar. — Forms a thick, shining, wavy, moist, 

 greenish yellow, iridescent expansion, the surface of which is pitted. Later it 

 becomes dirty yellow and opaque. It develops less well in the incubator. 



Blood Serum. — Forms a yellow moist shining expansion, which rapidly forms- 

 a gutter, and the greater part of the serum is subsequently Uquefied. It will 

 not develop in the incubator. 



Potatoes.— Grows slowly at first, but later forms a raised dark honey-yellow,. 

 dry, shining, rough expansion, which afterwards becomes yellowish brown and. 

 slimy. It will not develop in the incubator. 



Bboth. — Renders it turbid, forming a yellow deposit. In the incubator the: 

 liquid remains clear. 



H H 



