BACILLI 477 



BACILLUS CAKNICOLOE (' Fleischfarbiger bacillus ' ) 



LIQUEFIES GELATINE 



Authority. — Tils, ' Bakteriologische Untersuchung der Freiburger Leitungs- 

 wasser,' Zeitschrift f. Hygiene, vol. ix., 1890, p. 316. 



"Where Found. — In water supplied to Freiburg during the autumn months. 



Microscopic Appearance.—Slender bacillus about 2 fj. long and 0-5 fi broad. 

 Occurs usually singly. It is very motile. No spore formation observed. 



Cultures.— 



Gelatine Plates.— In two days round white colonies appear which form 

 cup-shaped liquefying depressions. Under a low power the circumference is- 

 sharply defined, and around the dark centre several light and dark circlets are 

 visible. 



Gelatine Tubes.— Grows rapidly all along the needle's path, and forms a 

 funnel-shaped liquefying depression, the lower part of which becomes filled 

 with faint pink-coloured masses of material, after which the liquefaction ceases. 



Potatoes. —Grows slowly, producing a dark flesh-coloured expansion. 



BACILLUS ACIDI LACTICI 



Authority. — Hueppe, Mitth. a. d, kais. Gcsundheitsamte, vol. ii., p. 337. 



"Where Found. — In sour milk. Found by Adametz in water, and also by 

 Tils {Zeitschrift filr Hygiene, vol. ix. p. 291). 



Microscopic Appearance. — Short and plump bacilli, from 1 to 1-7 ju long, 

 0*3 to 0'4 {x thick, half as wide as long ; occurs usually in pairs, rarely in chains 

 of four. Not motile. Produces spores at the poles. Is stained with methyl 

 violet, methylene blue, or Bismarck brown (Hueppe). 



Cultures. — 



Gelatine Plates. — Small white dots to begin with ; later grey white shining 

 centres, resembling bits of porcelain, and having a slightly irregular edge. 

 Under the microscope the surface colonies resemble small leaves spread out, 

 the centre of which are yellowish in colour, whilst the edge is thin and finely 

 serrated. 



Gelatine Tubes. — Along the needle track in the depth are seen tiny centres, 

 whilst later there appears on the surface a grey white shining, somewhat thick 

 and dry expansion. Does not liquefy the gelatine. 



Potatoes. — Yellow brown expansion. 



Milk. — Renders it acid. Precipitation of the casein. Produces gas. No 

 alcohol is produced. 



Remarks. — It will not grow below 10° C. in milk, requires from 10° to 12° C. At 

 15° C. acid is produced, which ceases between 45-3° and 45'5° C. (After eight days 

 the casein is separated out.) It will grow in the absence of oxygen. It is aerobic 

 and facultatively anaerobic. 



