BACILLI 479 



BACILLUS LACTIS VISCOSUS 



Authority. — Adametz, Landwirthschaftliche Jakrbiicher, 1890, p. 185. 



Where Found. — In river-water near Vienna receiving the waste water from 

 ■some factories. Especially in the Petersbach, where as many as from 100 to 

 200 per c.c. were detected. Also found in thick milk. 



Microscopic Appearance. — Short rods, easily mistaken for slightly elongated 

 cocci; surrounded by a capsule, which is particularly noticeable in milk 

 cultures. In this medium it is nearly as broad as long, about 1*5 fi long and 

 1*25 /i broad, or 1*05 ^ long and 0*8 /j. broad. It gives rise sometimes to 

 filaments composed of 3 to 6 individuals (milk). In old milk cultures it gives 

 rise to involution forms resembling budding yeast-cells. It is slightly motile 

 in young cultures. 



Cultures.— 



Gelatine -GLYCERINE Plates. — Forms at the end of three or four days small 

 dot-shaped colonies, which rapidly extend over the gelatine. They are irregular 

 in contour, with a serrated edge and a thick whitish centre ; the surface is 

 smooth, whilst the periphery is thin and almost transparent, with a distinct 

 opalescence. The depth colonies only grow very feebly. No liquefaction takes 

 "place. 



Agar-agar. — Produces a narrow whitish growth, the edge of which is at first 

 smooth, but becomes later finely serrated. 



Milk. — Sterilised milk becomes in four to six weeks viscous like honey, so 

 iibat it can be drawn out into long strings ; in unsterilised milk the cream only 

 becomes stringy, and such cream yields a white, gi'easy butter, which rapidly 

 spoils, owing to the numbers of the butyric acid bacillus present. Adametz 

 ■concludes that the B. lactis viscosus paves the way for the activity of the 

 butyric ferment. 



Remarks.— It grows rapidly at 28^ C, but only moderately well at 10° to 15° C. It 

 renders milk viscid and stringy in four weeks, due apparently to the swelling of the 

 ■capsules surrounding the bacilluB, and not to any fermentation process. 



B. ALBUS PUTIDUS (' Fauliger weisser Bacillus ') 



L'aUEFIES GELATINE 



Authority. — Maschek, Die Bakierien der Nutz- und Trinkwasser, Adametz 

 Tienna, 1888. 



Where Found. — In water. 



Microscopic Appearance. — Small bacillus forming threads. It is motile. 

 No spore formation observed. 



Cultures. — 



Gelatine Plates. — Round white colonies, which do not extend to any dis- 

 lance over the surface. Under a low power they appear light brown in colour, 

 surrounded by a bright zone, which is about 5 mm. broad at the end of five days. 

 Liquefies the gelatine rapidly all along the path of the needle. It gives rise to 

 a,n intensely putrid smell. 



Agar-agar. — Produces an expansion, but with nothing characteristic. 



Potatoes. — Grows rapidly, producing a slimy expansion. 



