504 MICRO-ORGANISMS IN WATER 



MICEOCOCCUS UEEAE (Pasteur) 



Authority. — Pasteur, Gornptes 7-endus, vol. 1., 1860. Van Tieghem, Compi, 

 rend.y vol. Iviii., 1864. Jacksch, Zeitschrift f. physiologische Chemie, vol. v., 

 1881, p. 395. Leube and Grasser, Vi?-chow's Archiv, vol. c, p. 556. 



"Where Found. — In decomposed ammoniacal urine, also in air. Found by 

 Tils {loc. cit.) in water. 



Microscopic Appearance. — Cocci from 0-8 to 1-0 fi in diameter ; occurs fre- 

 quently arranged as diplococci and in tetrads, also frequently in more or less 

 long chains. Grows also in the shape of zoogloea and rose-wreaths (Jacksch). 



Cultures. — 



Gelatine Plates. — Forms small white shining spots having a mother-of- 

 pearl lustre, and a smooth surface and sharply rounded contour. At the 

 ordinary temperature of a room they reach in ten days' time the size of a six- 

 pence and are slightly raised above the level of the gelatine, and resemble drops 

 of wax. No liquefaction takes place. 



Gelatine Tubes. — Forms a thin tough thread-like growth along the needle's 

 path in the depth. Old cultures have a smell of paste. 



It grows best in Jacksch's culture-fluid: in 1 litre ^\ grm. magnesium sul- 

 phate, i grm. acid potassium phosphate, 5 grms. sodium potassium tartrate, and 

 6 grms. urea. 



Bemarks. — According to Jacksch, the most favourable temperature for its 

 growth is 30° to 33° C. ; below 0° no growth takes place, but it can be kept for several 

 days at -13° C. without being destroyed. It converts urea into ammonium carbonate. 



MICEOCOCCUS VEESICOLOE 



Authority. — Fliigge, Die Mikroorganismen, 1886, p. 177. 



"Where Found. — Frequently in air. Found also frequently in water by Tils 

 (loc. cit.). 



Microscopic Appearance. — Small cocci, arranged in pairs or in aggregates. 



Cultures. — 



Gelatine Plates. — Forms in the depth white dot-shaped centres in twenty- 

 four hours, which in two days become yellow in colour. Under a low power 

 these are circular, smooth -rimmed, finely granular, and of an opaque yellowish 

 green colour. The surface colonies form large expansions, irregular in shape, 

 often four-cornered, with lobular projections. They are slimy, shining, and 

 yellowish green in colour, but in some lights they have a greenish or bluish 

 mother-of-pearl lustre. The centre of the colony is often raised. No lique- 

 faction takes place. 



Gelatine Tubes. — Forms a mother-of-pearl expansion with an irregular 

 and frayed edge. In the depth small ball-shaped, yellow colonies make their 

 appearance. 



