Second Importation of Calif ornian Ova. 3 5 



in hot weather. When the hatching was 

 nearly finished, a very marked difference was 

 observable in the number hatched in each 

 box. No. 1 had only about 1000 live fish out 

 of 7000 eggs. These eggs were on the top, 

 nearest the ice. The next layer, in No. 2, 

 had probably 3000 fish out of 7000 ova. 

 No. 3 was the best of all, and there were 

 probably 6000 live fish out of 7000 ova. 

 Nos. 4, 5, and 6 were pretty equal, and 

 hatched over 50 per cent. The eggs in 

 No. 7 hatched out much earlier, but the per- 

 centage of loss was above the average. 

 Box No. 8, received the lowest layer of eggs, 

 which hatched out very quickly, having the 

 warmest water, and produced probably 5000 

 fish. 



It will thus be seen that the different lots 

 of eggs, when treated exactly in the same 

 way, varied very materially in the number 

 hatched, showing that the causes of this 

 difference were to be looked for in the treat- 

 ment of the ova when first taken, or in the 

 mode of packing, rather than in their manage- 

 ment after their arrival in Australia. 



After the young fish were fairly hatched but 



