ISOLATION AOT) IDENTIFICATION. 



EXERCISE III. 



ISOLATION AND IDENTIFICATION OF SOME SOIL ORGANISMS 

 GHOWN ON MEDIA EMPLOYED IN PKEVIOUS EXERCISE. 



1. By means of a platinum needle inoculate tubes of ster- 

 ile bouillon from several well-isolated colonies, appearing on 

 the bouillon agar, synthetic agar and gelatin plates made in 

 the previous exercise; selecting those colonies most dissim- 

 ilar in appearance. 



2. Incubate 3-4 days at 20° C. 



3. Plate out these cultures by the usual dilution method, 

 using bouillon agar. 



4. When pure cultures are obtained describe and identify 

 the organisms by the usual morphological and cultural ex- 

 amination. 



