12 LABORATORY BACTERIOLOGY. 



EXBECISE VI. 



THE INFLDENCE OF MOISTURE ON THE AMMONIFICATION OF 

 DRIED BLOOD. 



1. Weigh oS fourteen 100 gram portions of air-dry, sieved 

 soil, in tumblers. 



2. Add 5 grams of dried Wood to each. 



3. Stir thoroughly by means of a sterile spatula. 



4. Add sterile water to the soil portions as follows : 



1 & 2 2 cc. 



3 & 4 4 cc. 



5 & 6 8 cc. 



7 & 8 12 cc. 



9 & 10 18 cc. 



11 & 12 24 cc. 



13 & 14 30 cc. 



5. Cover and incubate 7-8 days at 20° C. 



6. Determine the ammonia formed by transferring to 

 copper flasks, distilling and titrating in the usual way. 



