40 LABOEATORY BACTERIOLOGY. 



9. Add water and a little lime; filter, wash, make up to 

 some convenient Aohime and determine the nitrates present 

 in aliquots. 



10. Dry and grind the residues and determine the total 

 nitrogen present by the regular Kjeldahl method. 



11. From the results obtained calculate the per cent, of 

 the NaNOa denitrified and the effect of excessive moisture 

 or excessive organic matter on the loss of nitrogen. 



