ISOLATION" AND DESCRIPTION". 55 



EXEECISE XLIII. 



THE ISOLATION AND DESCRIPTION OF CLOSTRIDIUM 

 PASTEURIANUM. 



1. By means of a platinum loop make transfers from the 

 cultures obtained in the previous exercise (XLII.) into 

 tubes of sterile Winogradski's solution. 



2. Incubate under anaerobic conditions for 10-14 days at 

 20° C. 



3. Inoculate from the cultures obtained thus into tubes 

 of sterile water and from these dilutions into two tubes (in 

 series) of "Winogradski's agar, liquefied and cooled (40° C.) 

 and plate in the usual way. 



4. Incubate under anaerobic conditions for 7-10 days 

 at 20° C. 



5. Inoculate from several well-isolated colonies into tubes 

 of Winogradski's solution and incubate as before. 



6. If the cultures are not pure they should be plated out 

 as before until pure cultures are obtained. 



7. Describe the character of the growth on the various 

 media employed an the ordinary cultural examination of 

 organisms. 



8. Examine in stained and hanging-drop preparations for 

 morphological descriptions. 



