THE GEOWTH OF B. KADICICOLA. 59 



EXEECISE XLVI. 



THE GROWTH OE B. EADICICOLA ON VARIOUS CULTURE MEDIA. 



1. Inoculate from the pure cultures of B. Eadicicola ob- 

 tained from the various legumes in Exercise XLIV into 

 tubes of the following solutions : 



(1) "Radicicola" solution. 



(2) "Sugar-ash" solution. 



(3) "Modified Radicicola" solution. 



(4) "Radicicola" solution + .1 gm. (NH4)2S04. 



2. Incubate all the cultures 10-14 days at 20° C. 



3. Describe the growth of the organisms in the different 

 media. 



4. Examine in hanging-drop and stained preparations and 

 compare the size, motility, etc., of the organisms grown under 

 these varying conditions. 



