60 LABOKATOEY BACTERIOLOGY. 



BXEECISB XLVII. 



THE CULTIVATION OF. AMYLOBACTEE SPECIES. 



1. Boil 100 cc. of water in a tall, narrow beaker, until the 

 air is expelled. 



2. Add in small quantities at a time, coarsely powdered 

 rice, or coarsely powdered, barley. 



3. When the mass is thick enough to hold a spoon in an 

 upright position, stir in a small quantity of flour and incor- 

 porate it with the lower portion of the mass. 



4. Cover with a glass plate and keep at 35°-37° C. 



5. Fermentation will set in at 13-36 hours. 



6. By staining with a dilute potassium iodide solution, 

 granulose may be detected in many spindle-shaped sporan- 



