BUTYRIC I'EEMBNTATION. 01 



EXERCISE XLVIII. 



BUTYRIC FERMENTATION. 



1. Six 350 cc. Erlenmeyer flasks are filled almost full of 

 "synthetic" solution. 



2. Plug and sterilize as usual. 



3. Inoculate thus: 



1 & 2 — 0.5 cc. old milk. 

 3 & 4 — 0.5 gm. old cheese. 

 5 & 6 — 0.5 gm. old compost. 



4. Incubate 10-14 days at 20° C. 



5 Test qualitatively for butyric acid. 

 6. Examine the cultures obtained, in hanging-drop and 

 stained preparations and describe the organisms present. 



