f)3 LABOEATOEY BACTEEIOLOGY. 



EXEECISB XLIX. 



AEROBIC CELLULOSE FERMENTATION. 



1 . Make up a solution containing : 



1,000 cc. tap water. 

 1 gm. NH^Cl. 

 0.5 gm. KjHPO,. 



3. Weigh off three portions of 1-3 gms. filter paper. 



3. Place a piece of filter paper and 3.0 gms. CaCOg in 

 each of three large Petri dishes. Pour enough of the above 

 solution into each dish to cover the bottom completely. 



4. Add 3 cc. of an infusion of well-rotted manure to one 

 portion, and 3 gms. of garden soil to another portion. 



5. Open every second day and add vrater to maintain the 

 supply of moisture. 



6. Incubate 3-i weeks at 28°-35° C. 



7. Eemove the filter paper very carefully with forceps and 

 wash thoroughly. 



8. Dry carefully and weigh. 



9. The differences between the initial and final weights 

 will show the amount of filter paper decomposed by the 

 aerobic cellulose ferments. 





