64 LABORATOEY BAOTEEIOLOGY. 



EXEBCISE LI. 



ANAEKOBIC CELLULOSE EEE5IENXATI0N. 



1. Make up a solution of : 



1,000 cc. distilled water. 



1 gm. KJIPO,. 



1.0 gm. "(NH^)3(P0.),. 



0.5 gm. MgSO,. 



Traces of NaCl and Fe^Clo. 



3. Place in 900 cc. Eriennieyer flask 2 gms. of filter paper, 

 or of pure absorbent cotton, and 2 gms. of C'a(^0;,. 



3. Fill to the neck with the above solution. 



4. Inoculate with several cc. of infusion of well-rotted 

 horse manure. 



5. Incubate at 30°-35° for 4—6 weeks. 



6. Pour off liquid ; dry and weigh the cellulose. 



7. Eecord changed appearance and loss in weight. 



J.h7 



