ISOLATION AND DESCRIPTION. 65 



EXEECISB LII. 



THE ISOLATION AND DESCRIPTION OF CELLULOSE FERMENTS. 



1. From the cultures obtained in Exercises XLIX. and 

 L. inoculate by means of a platinum loop into tubes of 

 sterile water. 



2. Then inoculate into tubes of sterile "cellulose" agar in 

 series and plate in the usual way. 



3. Incubate 10-14 days at 20° C; the cultures obtained 

 from Exercise L. being kept under anaerobic conditions in a 

 Novy jar. 



4. Prom well isolated colonies inoculate into tubes of 

 sterile "cellulose" solution containing strips of filter paper, 

 tubes almost full of solution being used for the anaerobic 

 ferments. 



5. Microscopic hanging, drop and stained preparations are 

 made and careful comparative descriptions of the organisms 

 recorded. 



